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Bigj66

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Posts posted by Bigj66

  1. My oil cooler is part number PBC000020 (P38 after YA4455076) and the two connecting hoses are listed in the parts book as PBP500260 and PBP500270.

    I ordered two of the PBP500260 pipes as I just need the two cooler side female ends for my project. When they arrived they came with M18 female threaded nuts and not the M22 ones that the cooler has.

    There’s no sign of any adapter between the two in the parts manual.

    Can any P38 owners shed any light on the correct parts that I need?

  2. On 10/20/2020 at 7:29 PM, Geojake said:

    I know, that is the issue. I was hoping that someone on here may found a workaround.

    I can always attach the one support letter to the chassis and then try and find somewhere to weld a bracket to for the other but it won’t be easy. 

    Could you drill and rivinut the chassis to have a bolted connection for the bracket rather than a welded one?

    • Like 1
  3. Just revisiting this thread again as I want to order the fittings that I’ll need to make up the pipe work.

    What’s the purpose of using the adapter that’s fitted to the TDi radiator oil cooler?

    I measured the thread at M22 x 1.5 but with the pipe to the oil filter housing being 3/8 BSP then it obviously needs the adapter between the two but is there any reason why I can’t just connect an M22 AN fitting direct to the radiator if I’m not using the original steel pipe?

    Will it still seal or does it need a tapered end?

  4. 2 hours ago, Stellaghost said:

    Probably a very stupid suggestion but I have some radiator hose with smaller off take where your sender might be able to be jubilee clipped in your welcome to it if you think it's any good regards Stephen 

    20201008_181215.jpg

    20201008_181231.jpg

    Thanks Stephen but the hose layout is quite tight so they need to be as standard as possible to fit around the front of the engine.

    The thermostat is in a different location on the Thor so I’m hoping I can get the sender fitted in line with the outlet that’s attached to where the thermostat housing is on the earlier engines.

    82DAA7F4-5BE5-4355-B794-44E1067D781F.jpeg.5fd14aad24882650804932d798dbaa91.jpeg

    Alternatively, I could buy the MS thermostat housing adapter and fit a standard V8 outlet but I’m not sure whether the hole that’s tapped into it is too big for the series sender.

  5. 17 minutes ago, Red90 said:

    What are you trying to achieve?

    Nothing too difficult it’s just that the Thor V8 manifold doesn’t have a port for the series temperature sender so I just need to find or make an in line unit to keep everything standard with the gauge.

  6. Has anyone sent used Landrover parts to the US and can advise on the most cost effective method please?

    A chap in Texas has asked me if I could post my battery tray but a quick look on Parcel force etc is coming back with some frighteningly high quotes so before I give him the price I just want to check what others have done.

  7. 2 hours ago, monkie said:

    Thank you for the responses to my post. I'm pleased that it has given you an insight into what the media aren't interested in telling the public. I think a news special showing people what the inside of a molecular lab looks like or a day in the life of would be helpful. 

    Good question. You are already (I hope!) doing this in a way.

    Firstly for those who aren't sure let's call the virus by its correct name because the media often are not: Covid-19 is the term to used for the disease state or illness not the virus. Covid-19 is caused by the virus SARS-CoV-2 which is severe acute respiratory syndrome - Coronavirus 2. Just how AIDS is the disease but is caused by the virus HIV.

    Basically viruses contain their genetic material in a shell (capsid). The capsid contains proteins and a load of other stuff the virus needs do it's job and infect a host. The best way to picture the capsid on SARS-CoV-2 is like a fatty layer. 

    If you can open this layer then you have deactivated the virus i.e rendered it unable to function correctly. 

    As it is a fatty layer on SARS-CoV-2, soap will do this nicely. This is why it is very important to properly wash your hands as soap will break apart the virus's fatty layer rendering it useless or dead. 

    In the lab, because using a molecular technique (PCR) which is used to detect the presence of the virus by amplifying and detecting its nucleic acids (RNA in this case for SARS-CoV-2), it doesn't matter if the virus is dead or alive in terms of the test. 

    As respiratory viruses are spread to humans mainly by aerosol, the samples can be easily aerosoled by opening tubes out in the open in the lab to put on the instrument performing the test. For the safety of the lab staff they open the tubes in a special biological safety cabinet and either heat them (not a great method, so isn't often used in the UK) or add something called lysis buffer. Lysis buffer essentially does what soap does and destroys the viruses capsid. This kills the virus but importantly for PCR it does not destroy the RNA which then goes through a clean up process and then the amplification/detection process. 

    I hope this detail answers your question? 

    Great explanation, thanks for taking the time.

     

  8. On 9/22/2020 at 11:18 PM, monkie said:

    Again, please let me shed some light on the true situation regarding testing in hospital labs by removing any current politics. I work for one of the largest clinical diagnostics companies in the PCR section.

    The virus is detected (from swabs taken from the nose and throat) by PCR. Not all hospital labs are set up to do this specialist test. Let me assure you that the company I work for (and others) and NHS England have been putting additional capacity for this type of testing in place all throughout the summer as well additional biomedical scientist staff being retrained from other areas of pathology to do this testing.

    Most labs can only process a few thousand tests a day - a limitation of the technique of PCR. The PCR test is not quick but it is highly sensitive and specific. It can take anywhere between 6 and 2 hours from sample in to result out. This is because the sample itself can not undergo PCR straight away. Firstly the sample must be deactivated by heat or chemical treatment so it is safe for the lab staff to handle. This deactivated sample must be extracted - that is the virus (if present) chemically broken open (lysis) to release the RNA and then purified to remove it from substances that may interfer with the test. This "extracted" sample then can undergo the PCR test. If an analyser goes down (they are not designed to run 24/7 for months on end doing just one type of test) then the lab find it very difficult to catch up. They cannot accept more samples and therefore if you require a test you will not be able to get swabbed during this period, other than being offered a swab potentially miles away from your home.

    This is what happens at the moment when a hospital lab needs more equipment:

    NHS England decide if that lab in that geographical area needs the additional capacity over another area (the instruments and tests are in very limited supply as global demand is massive). The order is placed and it takes between 4-6 weeks to get the instrument shipped, delivered, alteration work to the lab (additional power supply, IT network points, moving walls, shelves benches etc) completed and the instrument placed in the lab. A further week or maybe 2 elapses whilst an engineer (in between dealing with breakdowns elsewhere) to install, calibrate and comission the instrument ready for use. Lab staff then take about 1 week to be trained and evaluate the test (to prove it works in their lab by testing known panels). They then get the green light to use the instrument for clinical diagnostic use. Fortunately it is not possible for any old idiot to set up a lab and start turning our tests, it is quite rightly a heavily regulated environment and a lab cannot just start a new type of test without providing evidence the test is reliably working without contamination or producing false results as a result of the lab staff not using the test and handling the sample correctly.

    To run the test, the instruments use >95% Ethanol, lysis buffers, wash buffers, elution buffers, RNase free water, SARS-CoV-2 primer/probes, control material, 1,000s and 1,000s and 1,000s of disposable pipette tips and loads more other consumable plasticwear. If any one of these items is in limited supply then the testing can not run at full capacity and decisions have to be made as to where to send the limited consumables as to best serve the hardest hit areas where testing demand is needed most.

    PCR is the gold standard of testing because it looks directly for the viral RNA (or DNA for some other viruses), it can detect as little as <100 bits or copies of viral RNA in 1ml of sample. The rapid tests that are talked about are starting to come through but so far the sensitivity of these tests lags far behind that of PCR which is why they are not being used. Antibody testing is different and is not used to diagnose a current infection. At the moment we don't know what having antibodies to SARS-CoV-2 means exactly so this testing has dropped off. Some people thought if they tested positive for antibodies they were immune so went back to life as normal, making the problem worse.

    I hope this illustrates that no matter what your political opinion and no matter who was running the country - nothing regarding testing would be different today in the middle of this pandemic. NHS pathology labs have been shaped by years of policy across several governments to consolidate testing to a few specialist labs (which in many ways makes sense and saves the NHS a lot of money). It is called the Carter review if you are interested and outlined how the NHS could save £0.2 billion by consolidating pathology.

    Excellent and very informative post. I wish someone would tell the media this although we should never let the truth get in the way of a good rumour 🙄

    I’m interested in this comment “Firstly the sample must be deactivated by heat or chemical treatment so it is safe for the lab staff to handle.”

    Can you explain this a bit more please? How does the deactivation process make the sample safe for the lab staff? Can/is this used elsewhere to prevent transmission?

    • Like 1
  9. 7 hours ago, uninformed said:

    Has anyone here looked into the Design and Developments Engineering kits? Should be available soon. 370 quid by the sounds of it. Looks like early 90/110 caliper

    Yes but as with the Zeus kit you can’t fit standard rims.

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